In gratitude of the loyal support of our members, the CNS is offering complimentary 2021 Annual Meeting registration to all members! Learn more.

  • Molecular Signatures of Carotid Plaques

    Final Number:
    5

    Authors:
    Georgios A Zenonos MD; Ellen Caparosa MD; Andrew J Sedgewick PhD; Ana-Carolina Igami Nakassa MD; William Lariviere PhD; Yin Zao; Diane Carisle PhD; Lucia Stefaneanu; Brian T. Jankowitz MD; Paul A. Gardner MD; Yue-Fang Chang PhD; William Laframboise; Panayiotis Benos; Robert Max Friedlander MD

    Study Design:
    Laboratory Investigation

    Subject Category:
    Basic Science

    Meeting: AANS/CNS Cerebrovascular Section 2018 Annual Meeting

    Introduction: Our ability to predict which atherosclerotic plaques are at higher risk to become symptomatic is limited, resulting in numerous unnecessary interventions. A better understanding of the molecular signatures associated with rupture-prone plaques, and the development of peripheral biomarkers could help guide management

    Methods: Thirty-eight carotid plaques (26 symptomatic, 12 asymptomatic), obtained from carotid endarterectomies at our institution, were dissected into pre-bifurcation, bifurcation, and post-bifurcation segments. All were used for protein expression. In addition, 9 symptomatic, and 9 asymptomatic plaques were used to identify mRNA:miRNA interactions in the post-bifurcation segments. Furthermore, peripheral blood from 9 patients with symptomatic plaques, and 9 patients with asymptomatic plaques, as well as 3 normal controls were used to evaluate peripheral miRNA expression.

    Results: Protein expression profiles revealed increased levels of IL-1ß , IL-6, IL-8, and cleaved caspace-3 in symptomatic compared to asymptomatic plaques, and in the distal, compared to the proximal plaque segments (post-bifurcation> bifurcation> pre-bifurcation). Further analysis of the post-bifurcation segments revealed three high-confidence miRNA:mRNA pair interactions (negatively correlated) that were differentially expressed between symptomatic and asymptomatic plaques: hsa-miR-214/APOD, hsa-miR-484/DACH1, hsa-miR-942/GPR56 (p < 0.001, correlation q < 0.05). Evaluation of peripheral blood miRNA expression revealed 7 miRNAs to be differentially expressed between symptomatic, and asymptomatic plaques, and 13 miRNAs to be differentially expressed between symptomatic plaques and controls.

    Conclusions: The current study identifies a number of novel potential biomarkers for carotid plaque vulnerability. Prospective evaluation of these markers in a large number of patients with carotid stenosis is warranted to establish their clinical applicability

    Patient Care: The current study identifies novel biomarkers of carotid plaque vulnerability, which could help risk stratify patients with carotid stenosis, and allow better patient selection for carotid revascularization procedures

    Learning Objectives: By the conclusion of this session, participants should be able to: 1) discuss the importance of biomarkers in the risk stratification of carotid stenosis, 2) describe some of the most important novel biomarkers presented in this study

    References:

We use cookies to improve the performance of our site, to analyze the traffic to our site, and to personalize your experience of the site. You can control cookies through your browser settings. Please find more information on the cookies used on our site. Privacy Policy