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  • Rapid and Ultra-sensitive intraoperative IDH mutation detection system in glial tumors

    Final Number:

    Timucin Avsar; Alihan Sursal; Gizem Turan; Berfu Nur Yigit; Deniz Altunsu; Kutay Cantasir; Turker Kilic MD; Melih Acar

    Study Design:
    Laboratory Investigation

    Subject Category:

    Meeting: Congress of Neurological Surgeons 2018 Annual Meeting - Late Breaking Science

    Introduction: IDH1/2 mutations are indicative of good prognosis upon treatment and they may have intraoperative importance for disease management. Intraoperative diagnosis became a new technical approach in glioma management. If a neurosurgeon would know the mutation status in IDH genes, he/she would change the extent of resection in tumor removal. Because mutated tumors responsive to chemo/radiotheraphy. We aimed to conduct a comprehensive IDH mutation analysis by using PCR based methodology during surgery.Our aim is to provide rapid, highly sensitive and specific intraoperative mutation detection system with robust accuracy.

    Methods: Amplification refractory mutation system (ARMS) was modified and optimized. The novelty of our methodology is adding an extra mismatch to increase specificity. ARMS was used to detect the presence of all common IDH1 and IDH2 mutations including, R132H/C/S/G and R172K/M/W mutations in 236 glioma sample selected from our tumor bank and obtained prospectively. All results were also evaluated by Sanger sequencing and immunohistochemistry (IHC). We also evaluated the diagnostic and screening potential of the test by comparing the methods.

    Results: By addition of the third mismatch to conventional ARMS we successfully detect IDH1 and IDH2 mutations by 100% coherency with Sanger sequencing. Comparison between IHC and 3 mismatch-ARMS showed, 3-mismatch ARMS is superior to IHC in terms of accuracy, sensitive and specificity. Based on our results, frequencies of IDH1/2mutations among different glioma grades are, 6.67%, 69.84%, 65.08% and %8.75 for grade I, II, III and IV respectively.

    Conclusions: 3-mismatch ARMS is a novel methodology with great potential to be used as a intra-operative diagnostic test. It can detect IDH mutations almost one hour without the need of sequencing with the ability to be adapted for detection of various mutations through easy primer design.

    Patient Care: It will enhance the neurosurgeons' treatment decision during surgery and facilitate the molecular diagnosis with greater accuracy, sensitivity and specificity.

    Learning Objectives: By the conclusion of this session, participants should be able to: 1) Describe the importance of intraoperative diagnosis in glial tumors, 2) Discuss, the availability of PCR based methodological approaches in intra-operative diagnosis 3) Identify 3 mismatched ARMS method as novel suitable method in glial tumor molecular diagnosis


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