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  • High Throughput Metabolite Profiling Identifies Plasma Anandamide as a Biomarker of Functional Outcome After Aneurysmal Subarachnoid Hemorrhage

    Final Number:

    Christopher James Stapleton MD; Hannah Irvine; Zoe Wolcott; Aman B. Patel MD; Jonathan Rosand; W. Taylor Kimberly

    Study Design:
    Laboratory Investigation

    Subject Category:

    Meeting: Congress of Neurological Surgeons 2017 Annual Meeting

    Introduction: The quantification of metabolites in plasma samples in patients with aneurysmal subarachnoid hemorrhage (aSAH) can highlight important alterations in critical metabolic pathways. As metabolites reflect changes associated with disease conditions, metabolite profiling (metabolomics) can identify candidate biomarkers for disease and potentially uncover pathways for intervention.

    Methods: We performed high throughput metabolite profiling (Figure) across a broad spectrum of chemical classes (173 metabolites) on plasma samples taken from 119 patients with aSAH. Samples were drawn at 3 time points following ictus: 2-4, 7-10, and 12-14 days. Univariate and logistic regression analyses were performed to examine the relation of each metabolite with multiple outcome variables, including short- and long-term functional outcome (modified Rankin Scale, mRS).

    Results: A good functional outcome (mRS 0-2) was found in 63.1% and 66.7% of patients at 30 and 90 days, respectively, following aSAH. Plasma concentrations of the endogenous cannabinoid anandamide during days 2-4 after aneurysmal SAH were decreased by 48.1% (P < 0.0001) and 57.6% (P < 0.0001) in patients with mRS 0-2 at 30 and 90 days, respectively. A similar statistical result was noted with plasma anandamide concentrations averaged across all time periods. Logistic regression further demonstrated that anandamide remained an independent predictor of functional outcome (30 days: P = 0.04; 90 days: P = 0.03), even after adjusting for other factors that influence outcome, including age, World Federation of Neurological Surgeons grade (WFNS), Fisher grade, and symptomatic vasospasm.

    Conclusions: Decreased plasma anandamide following aSAH predicts a good functional outcome at 30 and 90 days. While a role for anandamide in aneurysmal SAH has not been previously reported, elevated anandamide levels have been implicated in neuronal apoptosis and cerebral edema in the acutely injured brain. These data highlight the increasing capability of metabolomics techniques in profiling large-sized cohorts to illuminate novel markers of disease and potential metabolic regulators.

    Patient Care: Aneurysmal subarachnoid hemorrhage (aSAH) is a devastating neurological condition with poorly understood molecular and metabolic underpinnings. We leveraged high throughput metabolite profiling techniques to better understand the metabolic alterations seen in aSAH. Through this next generation approach, we identified plasma concentrations of the endogenous cannabinoid anandamide to be associated with functional outcome after aSAH. Future investigations into the mechanism of anandamide's role in aSAH may lead to the development of novel therapeutic interventions to improve recovery and outcome.

    Learning Objectives: By the conclusion of this session, participants should be able to: 1) Describe the importance of elucidating the molecular and metabolic underpinnings of aneurysmal subarachnoid hemorrhage (aSAH). 2) Postulate a mechanism by which anandamide might influence functional outcome following aSAH. 3) Identify a treatment strategy for improving functional outcomes following aSAH based on the metabolic disturbances uncovered with metabolic profiling.


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