Introduction: It is known that tumor mesenchymal stem-like cells (tMSLCs) can be isolated from high-grade gliomas and also play a role in glioma progression. Moreover, it has been reported that the success of tMSLCs isolation is a critical indicator of the overall survival of World Health Organization grade IV glioma patients. Despite these reports, attempts to isolate tMSLCs from surgical specimen are not always successful. Given that weights of surgical specimens from high-grade gliomas vary, we presumed that weightier surgical specimens would be better for tMSLC isolation.
Methods: To test this, we assessed 51 fresh, high-grade glioma specimens and divided them into two groups according to success or failure of tMSLCs isolation.
Results: We found no significant difference in the weights of specimens in the two groups. We further found no significant differences between the two groups in terms of patient age, sex, pathological diagnosis, tumor type, or molecular markers. We did, however, determine that the estimated optimal weight of samples for tMSLC isolation was 180 mg.
Conclusions: Collectively, our findings indicate that, contrary to our expectations, the efficiency of tMSLCs isolation was not related to the weight of surgical specimens.
Patient Care: It could help surgeon to determine the range of resection.
Learning Objectives: 1. The weight of the specimen and the eficiency of tMSLCs isolation are not relevant.
2. Contrary to common belief, the weight of the specimen is negative correlation with the efficienc of tMSLCs isolation.
3. As known as, isolation of tMSLCs is related with negative prognosis.