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  • Functional Modulation of the Blood Brain Barrier

    Final Number:
    221

    Authors:
    Geoffrey Stricsek MD; Michael J. Lang MD; Ashwini Dayal Sharan MD; Robert H. Rosenwasser MD, FACS, FAHA; Lorraine Iacovitti Ph.D.

    Study Design:
    Laboratory Investigation

    Subject Category:

    Meeting: Congress of Neurological Surgeons 2017 Annual Meeting

    Introduction: Delivery of therapeutic agents to the brain is constrained by the blood-brain barrier (BBB). Previous work (Yarnitsky) suggested BBB permeability was increased with stimulation of the sphenopalatine ganglion (SPG). However, their model looked at FITC-dextran signal in CSF superfusate, a reflection of epithelial tight junctions at the blood-CSF barrier, and quantified BBB permeability using Evans blue, a marker insufficient for this role (Saunders).

    Methods: Experiments were conducted in Sprague-Dawley rats using 70kDa FITC-dextran as a marker to quantify BBB permeability. Once anesthetized, the right femoral vein was exposed and catheterized. Next, SPG fibers were exposed behind the right eye and an electrode was hooked around those fibers. Stimulation occurred in blocks of 90 seconds of “on” time at 5 volts and 10Hz followed by 60 seconds of “off” time. Injection of 0.1mL of a 100mg/mL concentration of FITC coincided with each “on” stimulation cycle; a total of 1.0mL was injected per animal. Control animals were not stimulated, but had the same injection protocol as test animals. 90 seconds after the final injection, a blood sample was collected and the cerebrovasculature was flushed. Each brain was removed, equally divided, and homogenized. Post-centrifugation supernatant from blood and tissue homogenates was analyzed using fluorescent spectrometry. FITC concentrations were calculated using known standards. An uptake ratio was calculated by dividing sample FITC concentration by blood FITC concentration.

    Results: Data from 4 control animals and 4 test animals demonstrated a significantly greater uptake ratio in test brains compared with control brains (p=0.001). A significantly increased uptake ratio was also observed in both right and left hemispheres of test animals compared with controls (right, p=0.03; left, p=0.01).

    Conclusions: Stimulation of SPG fibers significantly increased BBB permeability in both cerebral hemispheres of test animals when compared with controls.

    Patient Care: Functional modulation of the blood-brain barrier can improve delivery of numerous therapeutic agents to the brain and aid in the treatment of primary and secondary intracranial malignancies, neurocognitive disorders such as Alzheimer's and Parkinson's, and potentially improve stem cell delivery for neural regeneration in patients who have had a stroke.

    Learning Objectives: By the conclusion of this session, participations should be able to: 1. Describe the anatomic basis of the blood-brain barrier 2. Discuss the role of SPG stimulation in increasing blood-brain barrier permeability 3. Discuss the role of SPG stimulation in improving existing therapeutic modalities

    References: Yarnitsky D, Gross Y, Lorian A, et al. Blood-brain barrier opened by stimulation of the parasympathetic sphenopalatine ganglion: a new method for macromolecule delivery to the brain. J Neurosurg. 2004;101:303-309. Saunders N, Dziegielewska K, Mellgard K, Habgood M. Markers for blood-brain barrier integrity: how appropriate is Evans blue in the twenty-first century and what are the alternatives? Front Neurosci. 2015;9.

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