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  • Confocal laser endomicroscopy for real time histomorphological diagnosis: Our clinical experience with 150 brain and spinal tumor cases.

    Final Number:

    Cleopatra Charalampaki MD, PhD

    Study Design:
    Clinical Trial

    Subject Category:

    Meeting: Congress of Neurological Surgeons 2014 Annual Meeting

    Introduction: Confocal laser endoscopy (CLE) is a novel technique permitting in vivo histologic imaging with miniaturized endoscopic probes at excellent resolution. Goal was: 1)to analyze the technical aspects of CLE technique 2)to investigate the integration of CLE in the neurosurgical daily workflow in the operation room, 3)to create an easily integration of the CLE technique into the neurosurgical daily routine used endoscopic setting and providing immediate and intraoperativ histopathologic diagnosis of the entire entity on real time, and 4) to evaluate CLE for in vivo diagnosis in different types and models of intracranial and intraspinal neoplasia.

    Methods: Fresh surgical biopsies of 150 intracranial and intraspinal lesions were examined to test the signal intensity and adequate contrast for CLE imaging after topical application of 0.1ml acriflavine. The lesions examined were different grades of glioma, meningiomas, craniopharyngiomas, different types of intrasellar lesions, acoustic neurinomas, different metastasis, medulloblastomas, epidermoid tumors, spinal and in brain located ependymomas. Reproducible and specific histomorphologic criteria was the ex vivo histopathologic gold standard staining.

    Results: CLE equipment was easily integrated into the daily routine. We additionally combined the CLE equipment, with the microscopes and endoscopes in a picture in picture modus. The insertion of the CLE optical probe into the working channel of conventional brain endoscopes was easy to performed. CLE yielded high-quality histomorphology of brain and spinal tumors. CLE discrimination of neoplastic tissue was easy to perform based on tissue and cellular architecture. The resemblance with histopathology was excellent.

    Conclusions: Confocal laser endomicroscopy allows immediate in vivo imaging of normal and neoplastic brain tissue at high resolution. It may become helpful to screen for tumor free margins not only for improving the real time histological definition of the tumor tissue, but also increasing the ability to accurate surgical resection of malignant brain tumors on a cellular level.

    Patient Care: If the surgeons will be able to see the borders between tumor and normal tissue than the surgeries will be more accurate with increasing the resection of tumor.

    Learning Objectives: Confocal endomicroscopy is a new technique which allows to see on a cellular level. This means that this technique opens new dimensions regarding optical imaging and the surgeons will be able to operate with much more accuracy then before.


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